Toru Hisabori
Toru Hisabori

Toru Hisabori

Professor, Chemical Resources Laboratory
PhD 1985, Waseda University
E-mail: thisabor(at)res.titech.ac.jp
Office: Room 209, R1-A building, Suzukakedai campus

Areas of Research: Plant Biochemistry, Bioenergetics.

Keywords: ATP synthase, thioredoxin, redox regulation, cyanobacteria

Research interest:

I am working on plant biochemistry for more than thirty years since I have jumped into the research life at the master course student time. Indeed I have spent ton of spinach leaves for my research to extract the proteins, and then changed the research targets to proteins from Avena sativa, Arabidopsis thaliana, and pea. When I moved to Tokyo Tech. in 1995, I have initiated the research with cyanobacteria and E. coli as well. Hence my laboratory is dealing with various organisms now. I am mainly focusing on regulation of the chloroplast-type ATP synthase, which is well-known as a molecular motor enzyme. In addition, I am very curious about the redox regulation system in photosynthetic organisms. These two topics were well combined to establish our newly launched CREST project using cyanobacteria supported by JST from 2011.

Selected publications

  1. Hara S, Tatenaka Y, Ohuchi Y, Hisabori T. Direct determination of the redox status of cysteine residues in proteins in vivo. Biochem Biophys Res Commun. 456, Issue 1, 2 January 2015, Pages 339–343 (2014)
  2. Yoshida K, Matsuoka Y, Hara S, Konno H, Hisabori T. Distinct Redox Behaviors of Chloroplast Thiol Enzymes and their Relationships with Photosynthetic Electron Transport in Arabidopsis thaliana. Plant Cell Physiol. 55(8):1415-1425 (2014)
  3. Yoshida K, Noguchi K, Motohashi K, Hisabori T. Systematic exploration of thioredoxin target proteins in plant mitochondria. Plant Cell Physiol. 54(6):875-892 (2013)
  4. Hara S, Nojima T, Yoshida M, Seio , Hisabori, T. DNA-Maleimide: an improved maleimide compound for electrophoresis-based titration of reactive thiols in a specific protein.
    Biochim. Biophys. Acta 1830(4):3077-3081 (2013)
  5. Sunamura E-I, Konno H, Imashimizu M, Mochimaru M, Hisabori T. A conformational change of the {gamma} subunit indirectly regulates the activity of cyanobacterial F1-ATPase. J. Biol. Chem. 287(46):38695-704 (2012)
  6. Imashimizu M, Bernat G, Sunamura EI, Broekmans M, Konno H, Isato K, Roegner M, Hisabori T. Regulation of FoF1-ATPase from Synechocystis sp. PCC 6803 by the {gamma} and {epsilon} subunits is significant for light/dark adaptation.J Biol Chem. 286(30):26595-26602 (2011)
  7. Konno H, Isu A, Kim Y, Murakami-Fuse T, Sugano Y, Hisabori T. Characterization of the relationship between ADP-inhibition and {epsilon}-inhibition in cyanobacterial F1-ATPase.J Biol Chem. 286(15):13423-13429 (2011)
  8. Kim Y, Konno H, Sugano Y, Hisabori T. Redox regulation of rotation of the cyanobacterial F1-ATPase containing thiol regulation switch. J. Biol. Chem. 286(11):9071-9078 (2011)
  9. Motohashi K, Hisabori T. CcdA Is a Thylakoid Membrane Protein Required for the Transfer of Reducing Equivalents from Stroma to Thylakoid Lumen in the Higher Plant Chloroplast. Antioxid Redox Signal. 13(8):1169-1176 (2010)
  10. Meiss E, Konno H, Groth G, Hisabori T. Molecular processes of inhibition and stimulation of ATP synthase caused by the phytotoxin tentoxin.J Biol Chem. 283, 24594-24599 (2008)
  11. Konno H, Murakami-Fuse, T, Fujii F, Koyama F, Ueoka-Nakanishi H, Pack C-G, Kinjo M, Hisabori T. The regulator of the F1 motor: Inhibition of rotation of cyanobacterial F1-ATPase by the  subunit. EMBO Journal, 25, 4596-4604 (2006)
  12. Motohashi, K., Kondoh, A., Stumpp, M. T., Hisabori, T. Comprehensive survey of proteins targeted by chloroplast thioredoxin. Proc. Natl. Acad. Sci. USA 98, 11224-11229 (2001)

Ken-ichi Wakabayashi
Ken-ichi Wakabayashi

Ken-ichi Wakabayashi

Associate Professor, Chemical Resources Laboratory
PhD 2001, University of Tokyo
E-mail: wakaba(at)res.titech.ac.jp
Office: Room 215, R1-A building, Suzukakedai campus

Areas of Research: Cell Motility, Biochemistry.

Keywords: Cilia, Flagella, Dynein, Chlamydomonas, Phototaxis, Redox regulation

Research interest:

I like observation of something moving under the microscope (such as microorganisms and motor proteins). Since I was an undergraduate student, I have been interested in regulatory mechanisms of ciliary/flagellar motility. Cilia/flagella are hair-like organelles that beat and generate fluid flow around cells. They are well known as swimming apparatus of unicellular organisms or sperms, and recently it turned out that various organs in human have motile cilia/flagella that play important roles for our health. For the research of cilia/flagella, the unicellular green alga Chlamydomonas reinhardtii is the best model organism. So far we have shown the effects of Ca2+ or redox (reduction-oxidation) on flagellar motility, mechanisms of how periodic structure of flagella is made, etc. Since I moved to Tokyo Tech in 2012, I started to focus on the regulatory mechanism of Chlamydomonas phototaxis: they quickly change their swimming direction in response to the change of the direction of incident light. Phototaxis is regulated by various signals including Ca2+, phosphorylation and redox poise, but molecular mechanisms in detail are still to be clarified. I am interested in how these signals are orchestrated to regulate flagellar behavior to move cells in proper directions. Through this research, I would like to clarify the common regulatory mechanisms underlying various cellular motilities.

Selected publications

  1. Owa, M., Furuta, A., Usukura, J., Arisaka F., King, S.M., Witman, G.B., Kamiya, R., and
    Wakabayashi, K. Cooperative binding of the outer arm docking complex underlies
    the regular arrangement of outer arm dynein in the axoneme
    Proc Natl Acad Sci U.S.A. 111:9461-6. (2014)
  2. Ide, T., Owa, M., King, S.M., Kamiya, R., Wakabayashi, K. Protein-Protein Interactions
    between Intermediate Chains and the Docking Complex of Chlamydomonas Flagellar
    Outer Arm Dynein
    FEBS Letters 587:2143-9. (2013)
  3. Engel, B.D., Ishikawa, H., Wemmer, K.A., Geimer, S., Wakabayashi, K., Hirono, M., Craige, B.,
    Pazour, G.J., Witman, G.B., Kamiya, R., and Marshall, W.F. The role of retrograde
    intraflagellar transport in flagellar assembly and function
    J Cell Biol 199:151-167. (2012)
  4. Wakabayashi, K. Regulation of axonemal outer-arm dyneins in cilia
    Dyneins: Structure, Biology and Disease, Elsevier Inc. pp.297-311 (2011)
  5. Wakabayashi, K., Misawa, Y., Mochiji, S., and Kamiya, R. Reduction-oxidation poise
    regulates the sign of phototaxis in Chlamydomonas reinhardtii
    Proc Natl Acad Sci U.S.A. 108:11280-4. (2011)
  6. Wakabayashi, K., and King, S.M. Modulation of Chlamydomonas reinhardtii Flagellar
    Motility by Redox Poise
    J Cell Biol 173:743-54. (2006)

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