Department of Bioengineering
Hiroyuki Kojima

Adjunct Professor
PhD 1985, The University of Tokyo
E-mail: Hiroyuki_kojima(at)ajinomoto.com
Website: -
Office: -
*note : Prof. H. Kojima doesn't continue his course here in 2016.

Areas of Research: Breeding of microbe, Development of fermentation process.

Keywords: Amino acids, Nucleoside, Fermentation, Breeding, Enzymatic conversion, Process Development.

Research interest:

 Industrial production of amino acids stated in 1909 and Fermentative production of amino acids stated in 1957. During the course of study of the mechanism of amino acids production, various kinds of amino acids could be produced by fermentation. The amino acids production technology has been developing as same as the recent progress of biochemistry and molecular biology. Recent development of genome analysis also expands the area of the technology. This technology made it possible to produce other material than amino acids. This fermentation production technology is friendly to the environment. It is very important to establish the fermentation production technology not only for amino acids but various kinds of materials.

Selected publications

  1. Asakura Y, Kojima H, Kobayashi I.
    Evolutionary genome engineering using a restriction-modification system.
    Nucleic Acids Res. 2011 Aug 12.
  2. Fukui K, Koseki C, Yamamoto Y, Nakamura J, Sasahara A, Yuji R, Hashiguchi K, Usuda Y, Matsui K, Kojima H, Abe K
    Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions.
    J Biotechnol. 2011 Jun 10;154(1):25-34.
    .
    Imaizumi A, Koseki C, Matsui K, Kojima H.
    Improved production of enzymes, which are expressed under the Pho regulon promoter, in the rmf gene (encoding ribosome modulation factor) disruptant of Escherichia coli
    Biosci Biotechnol Biochem. 2006 Apr;70(4):949-57.
    .
    Imaizumi A, Kojima H, Matsui K
    The effect of intracellular ppGpp levels on glutamate and lysine overproduction in Escherichia coli.
    . J Biotechnol. 2006 Sep 18;125(3):328-37. Epub 2006 Apr 18.
  3. Imaizumi A, Takikawa R, Koseki C, Usuda Y, Yasueda H, Kojima H, Matsui K, Sugimoto S Improved production of L-lysine by disruption of stationary phase-specific rmf gene in Escherichia coli.
    . J Biotechnol. 2005 Apr 20;117(1):111-8.
  4. Ogawa-Miyata Y, Kojima H, Sano K.
    Mutation analysis of the feedback inhibition site of aspartokinase III of Escherichia coli K-12 and its use in L-threonine production.
    Biosci Biotechnol Biochem. 2001 May;65(5):1149-54.
  5. Takesada H, Ebisawa K, Toyosaki H, Suzuki EI, Kawahara Y, Kojima H, Tanaka T.
    A convenient NMR method for in situ observation of aerobically cultured cells.
    J Biotechnol. 2000 Dec 28;84(3):231-6.
  6. Kikuchi Y, Kojima H, Tanaka T.
    Mutational analysis of the feedback sites of lysine-sensitive aspartokinase of Escherichia coli.
    FEMS Microbiol Lett. 1999 Apr 1;173(1):211-5.
  7. Kikuchi Y, Kojima H, Tanaka T, Takatsuka Y, Kamio Y.
    Characterization of a second lysine decarboxylase isolated from Escherichia coli.
    J Bacteriol. 1997 Jul;179(14):4486-92.
  8. Hashiguchi K, Kojima H, Sato K, Sano K.
    Effects of an Escherichia coli ilvA mutant gene encoding feedback-resistant threonine deaminase on L-isoleucine production by Brevibacterium flavum.
    Biosci Biotechnol Biochem. 1997 Jan;61(1):105-8.
  9. Yasueda H, Kikuchi Y, Kojima H, Nagase K
    In-vivo processing of the initiator methionine from recombinant methionyl human interleukin-6 synthesized in Escherichia coli overproducing aminopeptidase-P.
    . Appl Microbiol Biotechnol. 1991 Nov;36(2):211-5.

Patent applications

Filing No. Filing date Publish No. Publish Date Registration Registration date Description(Japanese)
2000-244921 2000/8/11 2002-051787 2002/2/19 4265093 2009/2/27 スレオニン及びイソロイシンの製造法
2001-553920 2000/1/21 WO01/53459 2001/7/26 4207426 2008/10/31 L-リジンの製造法
2003-195464 2003/7/11 2004-089188 2004/3/25 4483213 2010/4/2 発酵法による目的物質の製造法
2003-380971 2003/11/11 2004-173685 2004/6/24 4379089 2009/10/2 発酵法による目的物質の製造法
2004-248239 2004/8/27 2005-095169 2005/4/14 4575086 2010/8/27 コハク酸の製造方法
2004-340187 2004/11/25 2006-149214 2006/6/15 4595506 2010/10/1 L-アミノ酸生産菌及びL-アミノ酸の製造方法
2005-513981 2004/9/17 WO2005/
026349
2005/3/24 4619291 2010/11/5 非アミノ有機酸の製造方法
2006-513736 2005/5/20 WO2005/
113744
2005/12/1     コハク酸生産菌及びコハク酸の製造方法
2006-513737 2005/5/20 WO2005/
113745
2005/12/1     コハク酸生産菌及びコハク酸の製造方法
2007-540991 2006/10/17 WO2007/
046389
2007/4/26     コハク酸の製造方法
2008-502748 2007/2/23 WO2007/
099867
2007/9/7     有機酸生産菌及び有機酸の製造法
H02069246 1990/3/19 H03275631 1991/12/6     抗痴呆薬
H03014600 1991/2/5 H04211375 1992/8/3     合成遺伝子及びそれを用いたヒト血清アルブミンの製造法
H03100630 1991/2/5 H04248983 1992/9/4     発酵法による芳香族アミノ酸の製造法
H03196226 1991/5/2 H05236947 1993/9/17 3185261 2001/5/11 発酵法による芳香族アミノ酸の製造法
H03245291 1991/6/19 H05007491 1993/1/19 2077501 1996/8/9 温度感受性プラスミド
H03307614 1991/11/22 H05076352 1993/3/30 3060668 2000/4/28 発酵法によるL-フェニルアラニンの製造法
H05308397 1993/12/8 H07155184 1995/6/20     発酵法によるL-リジンの製造法
H06511924 1993/11/10 WO94/11517 1994/12/1 3331472 2002/7/26 発酵法によるL-スレオニンの製造法
H07005223 1995/1/17 H07203977 1995/8/8 2763054 1998/3/27 コリネ型細菌の遺伝子組換え方法
H07512515 1994/10/26 WO95/11985 WO95/11985 2817400 1998/8/21 物質の製造法
H07516087 1994/11/28 WO95/16042 1996/4/30 2926990 1999/5/14 発酵法によるL-リジンの製造法
H08272114 1996/10/15 H10113183 1998/5/6 4088982 2008/3/7 発酵法によるL-アミノ酸の製造法
H08517479 1995/12/5 WO96/17930 1997/12/22 3692538 2005/7/1 新規リジンデカルボキシラーゼ遺伝子及びL-リジンの製造法
H09502887 1996/3/14 WO96/41871 1998/8/25 4004065 2007/8/31 発酵法によるL-リジンの製造法
H10297404 1998/10/19 H11192088 1999/7/21 3132487 2000/11/24 発酵法によるL-リジンの製造法
H11030561 1999/2/8 H11285381 1999/10/19 3132497 2000/11/24 発酵法によるL-リジンの製造法
S62331374 1987/12/25 H02000458 1990/1/5 2536570 1996/7/8 発酵法によるL-イソロイシンの製造法

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