Tomoko Matsuda
Tomoko Matsuda

Associate Professor, Department of Bioengineering
PhD 2000, Kyoto University
E-mail: tmatsuda(at)
Office: Room913, J3 building, Suzukakedai campus

Areas of Research: Biocatalysis, Green Chemistry, Asymmetric Organic Synthesis.

Keywords: Enzyme, Alcohol dehydrogenase, Lipase, Carbon Dioxide, Supercritical Fluid, Green Chemistry, Chiral Synthesis.

Research interest:

 Because of homo-chirality in biology on the earth, which means amino acids, for example, are composed of only left-handed or L-isomer, the discovery and development of stereoselective organic synthetic methods for pharmaceutical and agrochemicals are very important. Chemical and biological catalysts have been developed throughout several decades. To synthesize them in environmentally friendly methods, we have been using enzymes as catalysts. Particularly, we have found that strains of Geotrichum candidum have many robust oxidoreductases with extremely excellent stereoselectivity. Moreover, we succeeded in reactions by the microorganisms in non-aqueous green solvents such as supercritical CO2.

 We also achieved the waste-minimization in large-scale production of chiral compounds with a flow system using liquid CO2 as a solvent and an immobilized lipase as a catalyst packed in a column reactor. Moreover, lipase catalyzed reactions were found to be effectively enhanced using a liquid CO2 medium when they were compared with those using organic solvents. Now, we are pursuing to elucidate the mechanism of the acceleration in enzymatic reactions caused by CO2. By these studies of green chemistry using enzyme and CO2, we hope to fulfill the role of scientist to protect environments for the bright future.


Selected publications

  1. H. N. Hoang, T. Matsuda, Liquid carbon dioxide as an effective solvent for immobilized Candida antarctica lipase B catalyzed transesterification, Tetrahedron Lett. in press
  2. T. Yamamoto, Y. Nakata, C. Cao, Y. Sugiyama, Y. Asanuma, S. Kanamaru, T. Matsuda, Acetophenone reductase with extreme stability against a high concentration of organic compounds or an elevated temperature, Appl. Microbiol. Biotechnol. 2013, 97, 10413-10421.
  3. C. Cao, T. Fukae, T. Yamamoto, S. Kanamaru, T. Matsuda, Purification and characterization of fluorinated ketone reductase from Geotrichum candidum NBRC 5767, Biochem. Eng. J. 2013, 76, 13-16.
  4. Y. Nakata, T. Fukae, R. Kanamori, S. Kanamaru, T. Matsuda, Purification and characterization of acetophenone reductase with excellent enantioselectivity from Geotrichum candidum NBRC 4597, Appl. Microbiol. Biotechnol. 2010, 86, 625-631.
  5. T. Matsuda, K. Watanabe, T. Harada, K. Nakamura, Y. Arita, Y. Misumi, S. Ichikawa, T. Ikariya, High-efficiency and minimum-waste continuous kinetic resolution of racemic alcohols by using lipase in supercritical carbon dioxide, Chem. Commun. 2004, 2286-2287.
  6. T. Matsuda, K. Watanabe, T. Kamitanaka, T. Harada, K. Nakamura, Biocatalytic reduction of ketones by a semi-continuous flow process using supercritical carbon dioxide, Chem. Commun. 2003, 1198-1199.
  7. T. Matsuda, Y. Ohashi, T. Harada, R. Yanagihara, T. Nagasawa, K. Nakamura, Conversion of pyrrole to pyrrole-2-carboxylate by cells of B. megaterium in supercritical CO2, Chem. Commun. 2001, 2194-2195.
  8. T. Matsuda, T. Harada, K. Nakamura, Alcohol dehydrogenase is active in supercritical carbon dioxide, Chem. Commun. 2000, 1367-1368.
  9. Book Edition: T. Matsuda, Ed. "Future directions in biocatalysis" Elsevier, Amsterdam, 2007.
  10. Review article: T. Matsuda, R. Yamanaka, K. Nakamura, Recent progress in biocatalysis for asymmetric oxidation and reduction, Tetrahedron: Asymmetry, 2009, 20, 513-557.
  11. Review article: T. Matsuda, T. Harada, K. Nakamura, Organic synthesis using enzymes in supercritical carbon dioxide, Green Chem., 2004, 6, 440-444.

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