Satoshi Murakami
Satoshi Murakami

Professor, Department of Life Science
PhD 1997, Osaka University
E-mail: murakami(at)bio.titech.ac.jp
Office: Room 904, J2 building, Suzukakedai campus

Areas of Research: Structural Biology, Protein crystallography

Keywords: Membrane Protein, Membrane Transport, Drug Efflux, Drug Resistance

Research interest:

  • 1) Structural analysis of membrane transporters
    X-ray crystallographic analysis of membrane transporters.
  • 2) Functional analysis of membrane transporters
    Molecular biological, biochemical and biophysical analysis of membrane transporters based on their molecular structures at atomic level.
  • 3) Over-expression, purification and crystallisation of membrane proteins.
    Methodological challenges and new approaches to research in membrane protein crystallography.

Selected publications

  1. Water-mediated recognition of simple alkyl chains by heart-type fatty-acid-binding protein. S. Matsuoka, S. Sugiyama, D. Matsuoka, M. Hirose, S. Lethu, H. Ano, T. Hara, O. Ichihara, S.Kimura, S. Murakami, H. Ishida, E. Mizohata, T. Inoue and M. Murata, Angew. Chem. Int. Ed. Engl., 54, 1508-11 (2015)
  2. Drug uptake pathways of multidrug transporter AcrB studied by molecular simulations and site-directed mutagenesis experiments. X-Q. Yao, N. Kimura, S. Murakami, S. Takada, J. Am. Chem. Soc., 135, 7474-85 (2013)
  3. Growth of protein crystals in hydrogels prevents osmotic shock. S. Sugiyama, M. Maruyama, G. Sazaki, M. Hirose, H. Adachi, K. Takano, S. Murakami, T. Inoue, Y. Mori, H. Matsumura, J. Am. Chem. Soc., 134, 5786-5789 (2012)
  4. Drug export and allosteric coupling in a multidrug transporter revealed by molecular simulations. X-Q. Yao, H. Kenzaki, S. Murakami, S. Takada, Nat. Commun., 1, 117 (2010)
  5. Dynamic nature of disulphide bond formation catalysts revealed by crystal structures of DsbB. K. Inaba, S. Murakami, A. Nakagawa, H. Iida, M. Kinjo, K. Ito, M. Suzuki, EMBO J., 28. 779-791 (2009)
  6. Crystal structures of DsbB- DsbA complex revealing a Cysteine relocation mechanism. K. Inaba, S. Murakami, M. Suzuki, A. Nakagawa, E. Yamashita K. Okada, K. Ito, Cell 127, 789-801 (2006)
  7. Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. S. Murakami, R. Nakashima, E. Yamashita, T. Matsumoto and A. Yamaguchi, Nature, 443, 173-179 (2006)
  8. Crystal structure of bacterial multidrug efflux transporter AcrB. S. Murakami, R. Nakashima, E. Yamashita and A. Yamaguchi, Nature, 419, 587-593 (2002)

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