Satoshi Nakamura
Satoshi Nakamura

Satoshi Nakamura

Professor, Department of Bioengineering
PhD 1989, Tokyo Institute of Technology
E-mail: snakamur(at)bio.titech.ac.jp
Office: Room 907, J2/J3 building, Suzukakedai campus

Areas of research: Genetic engineering, Protein engineering, Directed evolution

Keywords: Extremophiles, Extremozymes, Alkaliphiles, Halophiles, Thermophiles.

Selected publications

  1. Yang, Y., Yatsunami, R., Ando, A., Miyoko, N., Fukui, Takaichi, S., Nakamura, S. Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica. J. Bacteriol., 197, 1614-1623 (2015).
  2. Yatsunami, R., Ando, A., Yang, Y., Takaichi, S., Kohno, M., Matsumura, Y., Ikeda, H., Fukui, T., Nakasone, K., Fujita, N., Sekine, M., T. Takashina, T., Nakamura, S. Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica. Front. Microbiol., 5, Article 100 (2014).
  3. Onodera, M., Yatsunami, R., Tsukimura, W., Fukui, T., Nakasone, K., Takashina, T., Nakamura, S. Gene analysis, expression, and characterization of an intracellular a-amylase from the extremely halophilic archaeon Haloarcula japonica. Biosci. Biotechnol. Biochem. 77, 281-288 (2013)
  4. Uni, F., Lee, S., Yatsunami, R., Fukui, T., Nakamura, S. Mutational analysis of a CBM family 5 chitin-binding domain of an alkaline chitinase from Bacillus sp. J813. Biosci. Biotechnol. Biochem. 76, 530-535 (2012)
  5. Yazawa, R., Takakura, J., Sakata, T., Ihsanawati, Yatsunami, R., Fukui, T., Kumasaka, T., Tanaka N., Nakamura, S. A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1. Biosci. Bitechnol. Biochem. 75, 379-381 (2011)
  6. Tsukimura, W., Watanabe, K., Morokuma, C., Yatsunami, R., Fukui T., Nakamura, S. Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution, J. Jpn. Soc. Extr. 9, 15-18 (2010)
  7. M. Ito, N. Doukyu, I. Narumi, H. Higashibata, H. Tamegai, T. Kunieda, T. Ito, T. Sato and S. Nakamura. Extremophiles. Coronasha 2014.
  8. S. Nakamura, K. Nakasone and T. Takashina. Genetics and genomics of triangular disc-shaped halophilic archaeon Haloarcula japonica strain TR-1. "Extremophiles Handbook" (K. Horikoshi, ed.), Springer, Tokyo, 2011, pp. 363-381.
     

Rie Yatsunami
Rie Yatsunami

Rie Yatsunami

Associate Professor, Department of Bioengineering
PhD 2000, Tokyo Institute of Technology
E-mail: yatsunami.r.aa(at)m.titech.ac.jp
Office: Room 908, J2/J3 building, Suzukakedai campus

Areas of Research: Genetic engineering, Protein engineering, Directed evolution

Keywords: metabolic engineering, extremophiles, extremozymes

Selected publications

  1. K. Endo, K. Nagao, K. Toyama, S. Ishida, T. Fukazawa, R. Yatsunami, T. Fukui, X. Xing and S. Nakamura. "Cloning of a chitinase gene from newly isolated alkaliphilic Nocardiopsis sp. strain F96 and extracellular production of the recombinant protein in Escherichia coli" J. Jpn. Soc. Extremophiles, 14, 21-29, 2015.
  2. Y. Yang, R. Yatsunami, A. Ando, N. Miyoko,T. Fukui,S. Takaichi,and S. Nakamura. "Complete Biosynthetic Pathway of the C50 Carotenoid Bacterioruberin from Lycopene in the Extremely Halophilic Archaeon Haloarcula japonica" J. Bacteriol., 197(9), 1614-1623, 2015.
  3. R. Yatsunami, A. Ando, Y. Yang, S. Takaichi,M. Kohno, Y. Matsumura, H. Ikeda, T. Fukui, K. Nakasone, N. Fujita, M. Sekine, T. Takashina, S. Nakamura, "Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica" Front. Microbiol., 5, 100-105, 2014,. doi: 10.3389/fmicb.2014.00100
  4. T. Tadikara, T. Matsubara, Y. Kubota, T. Kosaka, T. Ozawa, W. Tsukimura, R. Yatsunami, T. Fukui, K. Nakasone, T. Takashina and S. Nakamura “Gene expression and characterization of aerotaxis transducer HemAT from extremely halohilic archaeon Haloarcula japonica TR-1” J. Jpn. Soc. Extremophiles.12(1), 29-32, 2013
  5. M. Onodera, R. Yatsunami, W. Tsukimura, T. Fukui, K. Nakasone, T. Takashina and S. Nakamura “Gene analysis, expression and characterization of an intracellular α-amylase from extremely halophilic archaeon Haloarcula japonicaBiosci. Biotechnol. Biochem., 77 (2), 281-288, 2013.
  6. F. Uni, S. Lee, R. Yatsuanmi, T. Fukui and S. Nakamura “Mutational Analysis of a CBM Family 5 Chitin-Binding Domain of an Alkaline Chitinase from Bacillus sp. J813” Biosci. Biotechnol. Biochem., 76 (3), 530-535, 2012.
  7. S. Chen, F. Ye, Y. Chen, Y. Chen, H. Zhao, R. Yatsunami, S. Nakamura, F. Arisaka and X.-H. Xing “Mechanism of the thermal inactivation of a MBP-fused heparinase I: Biochemical investigation and kinetic modeling” Biotechnol. Bioeng., 108, 1841-1851 (2011).
  8. R. Yazawa, J. Takakura, T. Sakata, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura. “A Calcium-Dependent Xylan- Binding Domain of Alkaline Xylanase from Alkaliphilic Bacillus sp. Strain 41M-1” Biosci. Biotechnol. Biochem., 75 (2), 379-381, 2011

Research interest:(Satoshi Nakamura , Rie Yatsunami)

 I am interested extremozymes and functional proteins produced by extremophiles, such as alkaliphiles, halophiles, thermophiles, using genetic engineering, protein engineering and directed evolution. Extremozymes and functional proteins include xylanase, chitinase, chitosanase, beta-1,3-glucanase, alpha-amylase, glucoamylase, 3-isopropylmalate dehydrogenase, arabinofuranosidase, ferredoxin, transducer, FtsZ, cruxrhodopsin, cruxhalorhodopsin, etc.

AFM image of a triangular disk-shaped extremely halophilic archaeon
AFM image of a triangular disk-shaped extremely halophilic archaeon


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