研究成果

最新の研究成果については、こちら をご参照ください。

【代表論文】

1. Regulation of chloroplast ATP synthase

  • [1] Buchert F, Konno H, Hisabori T. Redox regulation of CF1-ATPase involves interplay between the γ-subunit neck region and the turn region of the βDELSEED-loop. Biochim Biophys Acta. 2015 Apr-May;1847(4-5):441-50. doi:10.1016/j.bbabio.2015.01.013.
  • [2] Sunamura EI, Kamei T, Konno H, Tamaoki N, Hisabori T. Reversible control of F1-ATPase rotational motion using a photochromic ATP analog at the single molecule level. Biochem Biophys Res Commun. 2014 Mar 28;446(1):358-63. doi: 10.1016/j.bbrc.2014.02.117. [Epub ahead of print]
  • [3] Hisabori T, Sunamura EI, Kim Y, Konno H. The Chloroplast ATP Synthase Features the Characteristic Redox Regulation Machinery. Antioxid Redox Signal. 2013 Nov 20;19(15):1846-54. doi: 10.1089/ars.2012.5044.
  • [4] Sunamura E, Konno H, Imashimizu M, Mochimaru M, Hisabori T. A conformational change of the γ subunit indirectly regulates the activity of cyanobacterial F1-ATPase. J Biol Chem. 2012 Nov 9;287(46):38695-704. doi: 10.1074/jbc.M112.395053. Epub 2012 Sep 25.
  • [5] Konno H, Nakane T, Yoshida M, Ueoka-Nakanishi H, Hara S, Hisabori T. Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes. Plant Cell Physiol. 2012 Apr;53(4):626-34. doi: 10.1093/pcp/pcs018. Epub 2012 Feb 22.

2. Other F1-ATPases (with Prof. Yoshida's lab.)

  • [1] Usukura E, Suzuki T, Furuike S, Soga N, Saita E, Hisabori T, Kinosita K Jr, Yoshida M. Torque generation and utilization in motor enzyme F0F1-ATP synthase: half-torque F1 with short-sized pushrod helix and reduced ATP Synthesis by half-torque F0F1. J Biol Chem. 2012 Jan 13;287(3):1884-91. Epub 2011 Nov 28.
  • [2] Ohsakaya S, Fujikawa M, Hisabori T, Yoshida M. Knockdown of DAPIT (diabetes-associated protein in insulin-sensitive tissue) results in loss of ATP synthase in mitochondria. J Biol Chem. 2011 Jun 10;286(23):20292-6. Epub 2011 Feb 23.
  • [3] Hara, K. Y., Kato-Yamada, Y., Kikuchi, Y., Hisabori T., Yoshida, M. (2001) The role of the DELSEED motif of F1-ATPase: propagation of the inhibitory effect of the epsilon subunit. J. Biol. Chem. 276, 23969-73.
  • [4] Kato-Yamada Y, Yoshida M, Hisabori T. (2000) Movement of the helical domain of the epsilon subunit is required for the activation of thermophilic F1-ATPase. J. Biol. Chem. 275, 35746-35750
  • [5] Kato-Yamada Y, Bald D, Koike M, Motohashi K, Hisabori T, Yoshida M (1999) epsilon Subunit, an endogenous inhibitor of bacterial F1-ATPase, also inhibits FoF1-ATPase J. Biol. Chem. 274, 33991-33994

3. Thioredoxins

  • [1] Mihara S, Yoshida K, Higo A, Hisabori T. Functional Significance of NADPH-Thioredoxin Reductase C in the Antioxidant Defense System of Cyanobacterium Anabaena sp. PCC 7120. Plant Cell Physiol. 2016 Dec 22. pii: pcw182. doi: 10.1093/pcp/pcw182. [Epub ahead of print]
  • [2] Yoshida K, Hisabori T. Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113.
  • [3] Yutthanasirikul R, Nagano T, Jimbo H, Hihara Y, Kanamori T, Ueda T, Haruyama T, Konno H, Yoshida K, Hisabori T, Nishiyama Y. Oxidation of a Cysteine Residue in Elongation Factor EF-Tu Reversibly Inhibits Translation in the Cyanobacterium Synechocystis sp. PCC 6803. J Biol Chem. 2016 Mar 11;291(11):5860-70. doi:10.1074/jbc.M115.706424.
  • [4] Sugiura K, Nagai T, Nakano M, Ichinose H, Nakabayashi T, Ohta N, Hisabori T. Redox sensor proteins for highly sensitive direct imaging of intracellular redox state. Biochem Biophys Res Commun. 2015 Feb 13;457(3):242-8. doi:10.1016/j.bbrc.2014.12.095.
  • [5] Hara S, Tatenaka Y, Ohuchi Y, Hisabori T. Direct determination of the redox status of cysteine residues in proteins in vivo. Biochem Biophys Res Commun. 2015 Jan 2;456(1):339-43. doi: 10.1016/j.bbrc.2014.11.082.

4. Flagella

  • [1] Ueki N, Ide T, Mochiji S, Kobayashi Y, Tokutsu R, Ohnishi N, Yamaguchi K, Shigenobu S, Tanaka K, Minagawa J, Hisabori T, Hirono M, Wakabayashi KI. Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2016 May 10;113(19):5299-304. doi: 10.1073/pnas.1525538113.
  • [2] Ide T, Owa M, King SM, Kamiya R, Wakabayashi KI. Protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein. FEBS Lett. 2013 Jun 6. doi:pii: S0014-5793(13)00437-7. 10.1016/j.febslet.2013.05.058. [Epub ahead of print]
  • [3] Engel BD, Ishikawa H, Wemmer KA, Geimer S, Wakabayashi K, Hirono M, Craige B, Pazour GJ, Witman GB, Kamiya R, Marshall WF. The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol. 2012 Oct 1;199(1):151-67.
  • [4] Mochiji S, Wakabayashi K. Redox regulation of phototactic migration in the green alga Chlamydomonas reinhardtii and its possible application. Commun Integr Biol. 2012 Mar 1;5(2):196-8.
  • [5] VanderWaal KE, Yamamoto R, Wakabayashi K, Fox L, Kamiya R, Dutcher SK, Bayly PV, Sale WS, Porter ME. bop5 mutations reveal new roles for the IC138 phosphoprotein in the regulation of flagellar motility and asymmetric waveforms. Mol Biol Cell. 2011 Jun 22. doi:10.1091/mbc.E11-03-0270. [Epub ahead of print]

5. Others

  • [1] Higo A, Isu A, Fukaya Y, Hisabori T. Designing Synthetic Flexible Gene Regulation Networks Using RNA Devices in Cyanobacteria. ACS Synth Biol. 2016 Sep 22. [Epub ahead of print]
  • [2] Yoshida K, Hisabori T. Adenine nucleotide-dependent and redox-independent control of mitochondrial malate dehydrogenase activity in Arabidopsis thaliana. Biochim Biophys Acta. 2016 Mar 3. pii: S0005-2728(16)30047-0. doi:10.1016/j.bbabio.2016.03.001.
  • [3] Higo A, Isu A, Fukaya Y, Hisabori T. Efficient Gene Induction and Endogenous Gene Repression Systems for the Filamentous Cyanobacterium Anabaena sp. PCC 7120. Plant Cell Physiol. 2016 Feb;57(2):387-96. doi: 10.1093/pcp/pcv202.
  • [4] Yoshida T, Ogola HJ, Amano Y, Hisabori T, Ashida H, Sawa Y, Tsuge H, Sugano Y. Anabaena sp. DyP-type peroxidase is a tetramer consisting of two asymmetric dimers. Proteins. 2016 Jan;84(1):31-42. doi: 10.1002/prot.24952.
  • [5] Sugimoto K, Okegawa Y, Tohri A, Long TA, Covert SF, Hisabori T, Shikanai T. A single amino acid alteration in PGR5 confers resistance to antimycin A in cyclic electron transport around PSI. Plant Cell Physiol. 2013 Sep;54(9):1525-34. doi: 10.1093/pcp/pct098. Epub 2013 Jul 19.

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