研究成果

粂 昭苑 教授
【代表論文】

  • [1] Sakano D., Choi S, Kataoka M, Shiraki N, Uesugi M, Kume K, Kume S. Dopamine D2 receptor-mediated regulation of beta cell mass. Stem Cell Report 7, 95-109, 2016.
  • [2] Shahjalal HM, Shiraki N, Sakano D, Kikawa H, Ogaki S, Baba H, Kume K., Kume S. Generation of insulin-producing beta-like cells from human iPS cells in a defined and completely Xeno-free culture system. J. Mol. Cell Biol. 6, 394-408, 2014.
  • [3] Shiraki N., Shiraki Y., Tsuyama T., Obata F, Miura M, Nagae G, Aburatani H., Kume K, Endo F, Kume S*. Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells. Cell Metab. 19, 780-794, 2014.
  • [4] Sakano D, Shiraki N, Kikawa K, Yamazoe T, Kataoka M, Umeda K, Araki K, Mao D, Matsumoto S, Nakagata N, Andersson O, Stainier D, Endo F, Kume K,Uesugi M and Kume S. VMAT2 identified as a regulator of late-stage beta cell differentiation. Nat. Chem. Biol. 10, 141-148, 2014.
  • [5] Yamazoe T, Shiraki N, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Sasaki Y, Kume K and Shoen Kume. A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells. J. Cell Sci. 126, 5391-5399, 2013.
  • [6] Ogaki S, Shiraki N, Kume K, Kume S. Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages. Stem Cells 31, 1086-1096, 2013.
  • [7] Umeda K, Suzuki K, Yamazoe T, Shiraki N, Higuchi Y, Tokieda K, Kume K, Mitani K., Kume S. Albumin gene targeting in human embryonic stem cells and induced pluripotent stem cells with helper-dependent adenoviral vector to monitor hepatic differentiation. Stem Cell Res. 10, 179-194, 2013.
  • [8] Higuchi Y, Shiraki N., Yamane K, Qin Z, Mochitate K, Araki K., Senokuchi K, Yamagata K, Hara M, Kume K, and Kume S*. Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages. J. Cell Sci. 123, 2733-2742, 2010.
  • [9] Shiraki N, Umeda K, Sakashita N, Takeya M, Kume K and Kume S. Differentiation of mouse and human ES cells into hepatic lineages. Genes Cells 13, 731-746, 2008.
  • [10] Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, and Kume S. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells 26, 874-885, 2008.
  • [11] Rajagopal J, Anderson W J, Kume S, Martinez OI and Melton DA. Insulin staining of ES cell progeny from insulin uptake. Science 299, 363, 2003.
  • [12] Saneyoshi T, Kume S, Amasaki Y, and Mikoshiba K. The Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos. Nature 417, 295-299, 2002.
  • [13] Kume S, Muto A, Inoue T, Suga K, Okano H and Mikoshiba, K. Role of Inositol 1, 4, 5-trisphosphate receptor in ventral signaling in Xenopus embryos. Science 278, 1940-1943, 1997.
  • [14] Kume S, Muto A, Aruga J, Nakagawa T, Michikawa T, Furuichi T, Nakade S, Okano H and Mikoshiba K. The Xenopus InsP3 receptor: structure, function and localization in oocytes and eggs, Cell 73, 555-570, 1993.

【日本語総説】

  • [1] 白木伸明 粂昭苑「iPS細胞と肝胆膵疾患―膵臓・肝臓への分化―」GI Research第24巻2号30-36, 2016年
  • [2] 坂野大介 粂昭苑「膵β細胞の再生」Bio Clinic 31(3), 28-32, 2016 白木伸明 粂昭苑「幹細胞維持と分化におけるメチオニン代謝」『がん幹細胞―新しい医療を求めて―』(III.基礎研究動向) 日本臨床73巻5号 765-772, 2015
  • [3] 大垣総一郎 粂昭苑「ES/iPS細胞からの内胚葉組織形成」生化学 87巻5号573-581,2015年

白木 伸明 准教授
【代表論文】

  • [1] Shahjalal HM, Shiraki N, Sakano D, Kikawa K, Ogaki S, Baba H, Kume K, Kume S. Generation of insulin-producing β-like cells from human iPS cells in a defined and completely xeno-free culture system. J Mol Cell Biol. 2014 Oct;6(5):394-408.
  • [2] Shiraki N, Shiraki Y, Tsuyama T, Obata F, Miura M, Nagae G, Aburatani H, Kume K, Endo F, Kume S. Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells. Cell Metab. 2014 May 6;19(5):780-94.
  • [3] Sakano D, Shiraki N, Kikawa K, Yamazoe T, Kataoka M, Umeda K, Araki K, Mao D, Matsumoto S, Nakagata N, Andersson O, Stainier D, Endo F, Kume K, Uesugi M, Kume S. VMAT2 identified as a regulator of late-stage β-cell differentiation. Nat Chem Biol. 2014 Feb;10(2):141-8.
  • [4] Yamazoe T, Shiraki N, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Sasaki Y, Kume K, Kume S. A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells. J Cell Sci. 2013 Dec 1;126(Pt 23):5391-9.
  • [5] Ogaki S, Shiraki N, Kume K, Kume S. Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages. Stem Cells. 2013 Jun;31(6):1086-96.
  • [6] Shiraki N, Yamazoe T, Qin Z, Ohgomori K, Mochitate K, Kume K, Kume S. Efficient differentiation of embryonic stem cells into hepatic cells in vitro using a feeder-free basement membrane substratum. PLoS One. 2011;6(8):e24228.
  • [7] Shiraki N, Umeda K, Sakashita N, Takeya M, Kume K, Kume S. Differentiation of mouse and human embryonic stem cells into hepatic lineages. Genes Cells. 2008 Jul;13(7):731-46.
  • [8] Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells. 2008 Apr;26(4):874-85.

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