研究成果

大窪研では、得られた研究成果を積極的に学会や学術誌等で発表します。
所属する学生も、1年間に1回は国内外の学会で発表をしています。

【論文(査読あり)】

  • [1] A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds. K. Yamada, Y. Masaki, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine, Org Biomol Chem., 12, 2255-2262 (2014).
  • [2] Properties of 5- and/or 2-modified 2´-O-cyanoethyl uridine residue : 2´-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component. Y. Masaki, R. Miyasaka, K. Hirai, T. Kanamori, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine, Org Biomol Chem., 12, 1157-1162 (2014)
  • [3] Assembly of pyrene-modified DNA/RNA duplexes incorporating a G-rich single strand region. K. Seio, M. Tokugawa, H. Tsunoda, A. Ohkubo, H. Arisaka, M. Sekine, Bioorg Med Chem Lett., 23, 6822-6824 (2013).
  • [4] Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminus. Y. Iijima, S. Kojima, E. Kodama, S. Kurohagi, T. Kanamori, Y. Masaki, A. Ohkubo, M. Sekine, K. Seio, Org. Biomol. Chem., 11, 8276-8282 (2013).
  • [5] Chemical synthesis of U1 snRNA derivatives. A. Ohkubo, Y. Kondo, M. Suzuki, H. Kobayashi, T. Kanamori, Y. Masaki, K. Seio, K. Nagai, M. Sekine, Org. Lett., 15, 4386-4389 (2013).
  • [6] Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine. K. Seio, T. Kanamori, M. Tokugawa, H. Ohzeki, Y. Masaki, H. Tsunoda, A. Ohkubo, M. Sekine, Bioorg. Med. Chem., 21, 3197-3201 (2013).
  • [7] Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides. K. Yamada, A. Ohkubo, Y. Esaka, T. Kanamori, Y. Masaki, K. Seio, M. Sekine, Bioorg. Med. Chem. Lett., 23, 3448-3451 (2013).
  • [8] Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups. K. Yamada, Y. Hattori, T. Inde, T. Kanamori, A. Ohkubo, K. Seio, and M. Sekine, Bioorg. Med. Chem. Lett., 23, 776-778 (2013).
  • [9] Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2'-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component. Y. Masaki, R. Miyasaka, K. Hirai, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine, Chem. Comm., 48, 7313-7315 (2012).
  • [10] Synthesis and properties of cationic 2'-O-[N-(4-aminobutyl)carbamoyl] modified oligonucleotides. K. Seio, M. Tokugawa, T. Kanamori, H. Tsunoda, A. Ohkubo, M. Sekine, Bioorg. Med. Chem. Lett., 22, 2470-2473 (2012).
  • [11] Formation of new base pairs between inosine and 5-methyl-2-thiocytidine derivatives. A. Ohkubo, Y. Nishino, Y. Ito, H. Tsunoda, K. Seio, and M. Sekine, Org. Biomol. Chem., 10, 2008-2010 (2012).
  • [12] Development of an efficient method for phosphorodiamidate bond formation by using inorganic salts. T. Harakawa, H. Tsunoda, A. Ohkubo, K. Seio, and M. Sekine, Bioorg. Med. Chem. Lett., 22, 1445-1447 (2012).
  • [13] Synthesis of 5'-terminal capped oligonucleotide using O–N phosphoryl migration of phosphoramidite derivatives. A. Ohkubo, N. Tago, A. Yokouchi, Y. Nishino, K. Yamada, H. Tsunoda, K. Seio, M. Sekine, Org. Lett., 14, 10-13 (2012).
  • [14] Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents. K. Seio, S. Kurohagi, E. Kodama, H. Tsunoda, A. Ohkubo and M. Sekine, Org. Biomol. Chem., 10, 994-1006 (2012).
  • [15] DNA duplexes and triplex-forming oligodeoxynucleotides incorporating modified nucleosides forming stable and selective triplexes. T. Kanamori, Y. Masaki, M. Mizuta, H. Tsunoda, A. Ohkubo, M. Sekine, K. Seio, Org. Biomol. Chem., 10, 1007-1013 (2012).
  • [16] Stable triplex formation using the strong stacking effect of consecutive thionucleoside moieties. A. Ohkubo, Y. Nishino, A. Yokouchi, Y. Ito, Y. Noma, Y. Kakishima, Y. Masaki, H. Tsunoda, K. Seio, M. Sekine, Chem. Comm., 47, 12556-12558 (2011).
  • [17] Biochemical behavior of N-oxidized cytosine and adenine bases in DNA polymerase-mediated primer extension reactions. H. Tsunoda, T. Kudo, Y. Masaki, A. Ohkubo, K. Seio, M. Sekine, Nucleic Acids Res., 39, 2995-3004 (2011).
  • [18] Synthesis of 2'-O-[2-(N-Methylcarbamoyl)ethyl]ribonucleosides Using Oxa-Michael Reaction and Chemical and Biological Properties of Oligonucleotide Derivatives Incorporating These Modified Ribonucleosides. Y. Takeshi, N. Okaniwa, H. Saneyoshi, A. Ohkubo, K. Seio, T. Nagata, Y. Aoki, S. Takeda, M. Sekine, J. Org. Chem., 76, 3042-3053 (2011).
  • [19] Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine. S. Cao, I. Okamoto, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine, Tetrahedron Lett., 52, 407-410 (2011).
  • [20] Synthesis and hybridization properties of 2'-O-methylated oligoribonucleotides incorporating 2'-O-naphthyluridines. M. Sekine, Y. Oeda, Y. Iijima, H. Taguchi, A. Ohkubo, K. Seio, Org. Biomol. Chem., 9, 210-218 (2011).
  • [21] Synthesis of oligodeoxynucleotides using fully protected deoxynucleoside 3'-phosphoramidite building blocks and base recognition of oligodeoxynucleotides incorporating N3-cyano-ethylthymine. H. Tsunoda, T. Kudo, A. Ohkubo, K. Seio, M. Sekine, Molecules, 15, 7509-7531 (2010).
  • [22] Insights into the DNA stabilizing contributions of a bicyclic cytosine analogue: crystal structures of DNA duplexes containing 7,8-dihydropyrido [2,3-d]pyrimidin-2-one. E. C. M. Juan, S. Shimizu, X. Ma, T. Kurose, T. Haraguchi, F. Zhang, M. Tsunoda, A. Ohkubo, M. Sekine, T. Shibata, C. L. Millington, D. M. Williams, A. Takénaka, Nucleic Acids Res., 38, 6737-6745 (2010).
  • [23] Synthesis and hybridization properties of oligonucleotides incorporating Bi- and Tri-cyclic cytosine derivatives. A. Ohkubo, T. Sakaue, H. Tsunoda, K. Seio, M. Sekine, Chem. Lett., 39, 726-727 (2010).
  • [24] Synthesis of oligodeoxynucleotides using the oxidatively cleavable 4-methoxytritylthio (MMTrS) group for protection of the 5'-hydroxyl group. K. Seio, M. Shiraishi, E. Utagawa, A. Ohkubo, M. Sekine, New J. Chem., 34, 984-992 (2010).
  • [25] Oligonucleotide synthesis involving deprotection of amidine-type protecting groups for nucleobases under acidic conditions. A. Ohkubo, Y. Kuwayama, Y. Nishino, H. Tsunoda, K. Seio, M. Sekine, Org. Lett., 12, 2496-2499 (2010).
  • [26] Linear relationship between deformability and thermal stability of 2'-O-modified RNA hetero duplexes. Y. Masaki, H. A. Ohkubo, K. Seio, M. Sekine, J. Phys. Chem. B., 114, 2517-2524 (2010).
  • [27] Synthesis of 6-N-(benzothiazol-2-yl)deoxyadenosine and its exciton-coupled circular dichroism. Y. Masaki, H. A. Ohkubo, K. Seio, M. Sekine, Bioorg. Med. Chem., 18, 567-572 (2010).
  • [28] Microwave-assisted synthesis of 2'-O-aryluridine derivatives. Y. Oeda, Y. Iijima, H. Taguchi, A. Ohkubo, K. Seio, M. Sekine, Org. Lett., 11, 5682-5585 (2009).
  • [29] Synthesis and hybridization of 2'-O-methyl-RNAs incorporating 2'-O-carbamoyluridine and unique participation of the carbamoyl group in U-G base pair. K. Seio, R. Tawarada, T. Sasami, M. Serizawa, M. Ise, A. Ohkubo, M. Sekine, Bioorg. Med. Chem., 17, 7275-7280 (2009).
  • [30] Synthesis and properties of nucleoside derivatives acylated by chemically stable 2-(trimethylsilyl)benzoyl group. K. Yamada, H. Taguchi, A. Ohkubo, K. Seio, M. Sekine, Bioorg. Med. Chem., 17, 5928-5932 (2009).
  • [31] Fluorescence properties of pyrimidopyrimidoindole nucleoside dC(PPI) incorporated into oligodeoxynucleotides. M. Mizuta, K. Seio, A. Ohkubo, M. Sekine, J. Phys. Chem. B., 113, 9562-9569 (2009).
  • [32] Efficient solid-phase synthesis of oligodeoxynucleotides having a 5'-terminal 2,2,7-trimethylguanosine pyrophosphate linkage. A. Ohkubo, R. Kasuya, K. Aoki, A. Kobori, H. Taguchi, K. Seio, M. Sekine, Bioorg. Med. Chem., 17, 4819-4824 (2009).
  • [33] Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs. K. Seio, Y. Takaku, K. Miyazaki, S. kurohagi, Y. Masaki, A. Ohkubo, M. Sekine, Org. Biomol. Chem., 7, 2440-2451 (2009).
  • [34] Computational evaluation of the stability of 2'-O-methyl-RNA/RNA duplexes incorporating 3-deazaguanine derivatives by ab initio calculations and a molecular dynamics simulation. T. Sasami, R. Tawarada, A. Ohkubo, M. Sekine, K. Seio, J. Mol. Struct. (Theochem), 899, 54-60 (2009).
  • [35] Introduction of 3'-terminal nucleosides having a silyl-type linker into polymer supports without base protection. A. Ohkubo, Y. Noma, K. Aoki, H. Tsunoda, K. Seio, M. Sekine, J. Org. Chem., 74, 2817-2823 (2009).
  • [36] Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine. K. Miyata, R. tamamushi, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine, Org. Lett., 11, 605-608 (2009).
  • [37] New thermolytic carbamoyl groups for the protection of nucleobases. A. Ohkubo, R. Kasuya, K. Miyata, H. Tsunoda, K. Seio, M. Sekine, Org. Biomol. Chem., 7, 687-694 (2009).
  • [38] Synthesis of oligodeoxynucleotides incorporating 2-N-carbamoylguanine and evaluation of the hybridization properties. T. Sasami, Y. Odawara, A. Ohkubo, K. Seio, M. Sekine; Bioorg. Med. Chem., 16, 8287-8293 (2008).
  • [39] Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues. K. Seio, M. Mizuta, K. Tasaki, K. Tamaki, A. Ohkubo, M. Sekine. Bioorg. Med. Chem., 16, 8287-8293 (2008).
  • [40] New nucleotide pairs for stable DNA triplexes stabilized by stacking interaction. M. Mizuta, J. Banba, T. Kanamori, R. Tawarada, A. Ohkubo, M. Sekine, K. Seio, J. Am. Chem. Soc., 130, 9622-9623 (2008).
  • [41] O-selective condensation using P-N bond cleavage in RNA synthesis without base protection. A. Ohkubo, Y. Kuwayama, T. Kudo, H. Tsunoda, K. Seio, M. Sekine, Org. Lett., 10, 2793-2796 (2008).
  • [42] Synthesis and hybridization properties of 2'-O-(tetrazol-5-yl)ethyl-modified oligonucleotides. H. Saneyoshi, K. Tamaki, A. Ohkubo, K. Seio, M. Sekine, Tetrahedron, 64, 4370-4376. (2008)
  • [43] Efficient synthesis of functionalized oligodeoxyribonucleotides with base-labile groups using a new silyl linker. A. Ohkubo, R. Kasuya, K. Aoki, A. Kobori, H. Taguchi, K. Seio, M. Sekine, Bioorg. Med. Chem., 16, 5345-5351 (2008).
  • [44] "Protected DNA Probes (PDP)" capable of strong hybridization without removal of base protecting groups. A. Ohkubo, R. Kasuya, K. Sakamoto, K. Miyata, H. Taguchi, H. Nagasawa, T. Tsukahara, T. Watanobe, Y. Maki, K. Seio, M. Sekine, Nucleic Acids Res., 36, 1952-1964 (2008).
  • [45] Synthesis and properties of DNA oligomers containing 2'-deoxynucleoside-N-oxide derivatives. H. Tsunoda, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine, J. Org. Chem., 73, 1217-1224 (2008).
  • [46] Synthesis and properties of oligodeoxynucleotides containing 5-carboxy-2'-deoxycytidines: M. Sumino, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine, Bioorg. Med. Chem. Lett., 18, 274-277 (2008).
  • [47] A pyrimidopyrimidoindole nucleoside (dC(PPI)): Photophysical properties and thermal stability of the modified DNA duplexes. M. Mizuta, K. Seio, K. Miyata, A. Ohkubo, H. Taguchi, M. Sekine, Nucleosides, Nucleotides, Nucleic Acids, 26, 1335-1338 (2007).
  • [48] Facile synthesis of 2'-O-cyanoethyluridine by ring-opening reaction of 2,2'-anhydrouridine with cyanoethyl trimethylsilyl ether in the presence of BF3 center dot Et2O. H. Saneyoshi, I, Okamoto, Y. Masaki, A. Ohkubo, K. Seio, M. Sekine, Tetrahedron. Lett., 48, 8554-8557 (2007).
  • [49] Synthesis and hybridization properties of oligodeoxynucleotides with long-chain linker. K. Seio, T. Terada, M. Mizuta, A. Ohkubo, H. Taguchi, M. Sekine, Helvetica Chemica. Acta., 90, 1946-1965 (2007).
  • [50] Synthesis of branched oligonucleotides with three different sequences using an oxidatively removable tritylthio group. E. Utagawa, A. Ohkubo, M. Sekine, K. Seio, J. Org. Chem., 72, 8259-8266 (2007).
  • [51] An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis. A. Ohkubo, H. Taguchi, K. Seio, H. Nagasawa, T. Tsukahara, M. Sekine, Bioorg. Med. Chem. Lett., 17, 5969-5973 (2007).
  • [52] Synthesis and hybridization properties of oligodeoxynucleotides incorporating 2-N-carbamoylguanine derivatives as guanine analogs. T. Sasami, Y, Odawara, A. Ohkubo, M. Sekine, K. Seio, Tetrahedron. Lett., 48, 5325-5329 (2007).
  • [53] A new hydrophobic linker effective for the in situ synthesis of DNA–CPG conjugates as tools for SNP analysis. A. Ohkubo, H. Taguchi, K. Seio, H. Nagasawa, T. Tsukahara, M. Sekine, Tetrahedron. Lett., 48, 5147-5150 (2007).
  • [54] Highly selective recognition of cytosine over uracil and adenine by a guanine analogue, 2-N-acetyl-3-deazaguanine, in 2'-O-methyl-RNA/RNA and DNA duplexes. K. Seio, T. Sasami, A. Ohkubo, K. Ando, M. Sekine, J. Am. Chem. Soc., 129, 1026-1027 (2007).
  • [55] Synthesis and fluorescent properties of bi- and tricyclic 4-N-carbamoyldeoxycytidine derivatives. K. Miyata, R. Mineo R. Tamamushi, M. Mizuta, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine, J Org Chem., 72, 102-108 (2007).
  • [56] Conformational studies of 4-N-carbamoyldeoxycytidine derivatives and synthesis and hybridization properties of oligodeoxyribonucleotides incorporating these modified bases. K. Miyata, R. Tamamushi, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine, Eur. J. Org. Chem., 16, 3626-3637 (2006).
  • [57] Synthesis and biological properties of new phosmidosine analogs having an N-acylsulfamate linkage. H. Taguchi, A, Ohkubo, K. Seio, H. Kakeya, H. Osada, T. Sasaki, M. Sekine, Nucleosides, Nucleotides, Nucleic Acids, 25, 647-654 (2006).
  • [58] Synthesis and properties of a new fluorescent bicyclic 4-N-carbamoyldeoxycytidine derivative. K. Miyata, R. Tamamushi, A. Ohkubo, H. Taguchi, K. Seio, T. Santa, M. Sekine, Org. Lett., 8, 1545-1548 (2006).
  • [59] Synthesis of oligodeoxyribonucleotides containing hydroxymethylphosphonate bonds in the phosphoramidite method and their hybridization properties. A. Ohkubo, K. Aoki, Y. Ezawa, Y. Sato, H. Taguchi, K. Seio, M. Sekine, Tetrahedron. Lett., 46, 8953-8957 (2005).
  • [60] Convenient synthesis of N-unprotected deoxynucleoside 3'-phosphoramidite building blocks by selective deacylation of N-acylated species and their facile conversion to other N-functionalized derivatives. A. Ohkubo, K. Sakamoto, K. Miyata, H. Taguchi, K. Seio, M. Sekine, Org. Lett., 7, 5389-5392 (2005).
  • [61] Chemically stabilized phenylboranylidene groups having a dimethoxytrityl group as a colorimetrically detectable protecting group designed for cis-1,2-diol functions of ribonucleosides in the Solid-Phase Synthesis of m22,2G5'ppT. M. Sekine, M. Aoyagi, M. Ushioda, A. Ohkubo, K, Seio, J. Org. Chem., 70, 8400-8408 (2005).
  • [62] Synthesis and hybridization affinity of oligodeoxyribonucleosides incorporating 4-N-(N-arylcarbamoyl)deoxycytidine derivatives. K. Miyata, R. Tamamushi, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine, Tetrahedron Lett., 45, 9365-9368 (2004).
  • [63] O-Selectivity and utility of phosphorylation mediated by a phosphite intermediate in N-unprotected phosphoramidite method. A. Ohkubo, K. Seio, M. Sekine, J. Am. Chem. Soc., 126, 10884-10896 (2004).
  • [64] A new approach for pyrophosphate bond formation starting from phosphoramidite derivatives by use of 6-trifluoromethyl-1-hyroxybenzotriazole-mediated O-N phosphoryl migration. A. Ohkubo, K, Seio, M. Sekine, Tetrahedron Lett., 45, 979-982 (2004).
  • [65] A new strategy for the synthesis of oligodeoxynucleptides directed toward perfect O-selective internucleotidic bond formation without base protection. A. Ohkubo, K, Seio, M. Sekine, Tetrahedron Lett., 45, 363-366 (2004).
  • [66] Proton-Block strategy for the synthesis of oligodeoxynucleotides without base protection, capping reaction, and P-N bond cleavage reaction. M. Sekine, A. Ohkubo, K, Seio, J. Org. Chem., 68, 5478-5492 (2003).
  • [67] 2-(Azidomethyl)benzoyl as a new protecting group in nucleosides. T. Wada, A. Ohkubo, A. Mochizuki, M. Sekine, Tetrahedron Lett., 42, 1069-1072 (2001).

【総説、著書】

  • [1] 精密ゲノム遺伝子解析法を指向した新技術の展開; 関根光雄、大窪章寛、岡本到、小堀哲生、清尾康志:蛋白質 核酸 酵素2004, 49, 2978-2985.
  • [2] 保護プローブ型DNAチップ: 関根光雄、大窪章寛、岡本到、清尾康志: “ファイバー”スーパーバイオミメティックス(本宮達也 監修; NTC Inc.)2006, 第四編、第二章、第二節
  • [3] Ohkubo. A; Seio, K.;  Sekine, M.; DNA Synthesis Without Base Protection Using the Phosphoramidite Approach, Current Protocols in Nucleic Acid Chemistry,  3.15. 1-20. (2006)
  • [4] 人工塩基の高精度塩基識別能力を利用した遺伝子検出技術; 大窪章寛; 新しいDNAチップの科学と応用(関根光雄編;講談社サイエンティフィク)2007, 103-115.

【特許リスト】

  • 1.【発明の名称】「ヌクレオシドトリホスフェート誘導体」
    【出願番号】特願2008-057295
    【特許出願日】2008.03.07
    【公開番号】特開2009-215171(公開日2009.9.24)
    【出願人】国立大学法人東京工業大学
    【発明者】関根光雄、清尾康志、大窪章寛、角田浩佑、工藤智美
  • 2.【発明の名称】オリゴヌクレオチド固定化固相担体*
    【出願番号】特願2006-066396 大学出願整理No:05T285P
    【特許出願日】2006.3.10
    【PCT出願番号】PCT/JP2007/054645
    【PCT出願日】2007-0309
    【国際公開番号】WO/2007/105623(公開日:20.09.2007)
    【出願人】国立大学法人東京工業大学
    【発明者】関根光雄、清尾康志、大窪章寛、田中邦彦
  • 3.【発明の名称】塩基部無保護ホスホロアミダイト化合物の製造方法
    【出願番号】特願2005-225738
    【特許出願日】2005.8.3
    【公開番号】特開2007-039385(公開日2007.2.15)
    【出願人】シグマアルドリッチジャパン(株)
    【発明者】関根光雄,大窪章寛,清尾康志
  • 4.【発明の名称】固体支持体及びDNAチップ
    【出願番号】特願2005-263722
    【特許出願日】2005.9.12
    【公開番号】特開2007-078399(公開日2007.3.29)
    【出願人】国立大学法人東京工業大学
    【発明者】関根光雄,大窪章寛,清尾康志
  • 5.【発明の名称】トリチル型化合物
    【出願番号】特願2005-066555
    【特許出願日】2005.3.10
    【公開番号】特開2006-248956(公開日2006.9.21)
    【出願人】国立大学法人東京工業大学
    【発明者】関根光雄,清尾康志,大窪章寛,粕谷林太郎
  • 6.【発明の名称】オリゴヌクレオチド誘導体,遺伝子検出用プローブ及びDNAチップ
    【出願番号】特願2005-053417
    【特許出願日】2005.2.28
    【PCT出願番号】PCT/JP2006/303772
    【PCT出願日】2006.2.28
    【国際公開番号】WO2006/093157(公開日:2006.9.8)
    【出願人】国立大学法人東京工業大学
    【発明者】関根光雄,清尾康志,大窪章寛,坂本一石,佐々見武志
  • 7.【発明の名称】ホスホロアミダイドを含む3'末端ヌクレオシドユニットPreparation of 3'-terminal nucleoside unit containing diisopropylsilylbenzyl phosphoramidite and solid phase synthesis of nucleic acid oligomers
    【出願番号】特願2004-49312
    【特許出願日】2004.2.25
    【PCT出願番号】PCT2005-JP2058
    【PCT出願日】2005-0210
    【国際公開番号】WO /2005/080411(公開日:2005-9.1)
    【出願人】独立行政法人科学技術振興機構
    【発明者】関根光雄、清尾康志、大窪章寛
  • 8.【発明の名称】核酸固相合成用シリルリンカー
    Preparation of (silylbenzoylamino)alkanoic acid derivative as silyl linker for solid-phase synthesis of nucleic acid
    【出願番号】特願2004-49303
    【特許出願日】2004.2.25
    【PCT出願番号】PCT/2005/2059
    【PCT出願日】2005-0210
    【国際公開番号】WO 2005/080404(公開日:2005-9.1)
    【出願人】独立行政法人科学技術振興機構
    【発明者】関根光雄、清尾康志、大窪章寛
  • 9.【発明の名称】塩基部無保護による新規核酸合成法
    Novel method of synthesizing nucleic acid without protecting nucleotide bases
    【出願番号】特願2004-56707
    【特許出願日】2004.3.1
    【PCT出願番号】PCT/JP/2005/003053
    【PCT出願日】2005-0224
    【国際公開番号】WO2005-082923(公開日:2005-9.9)
    【出願人】独立行政法人科学技術振興機構
    【発明者】関根光雄、清尾康志、大窪章寛
  • 10.【発明の名称】ポリリン酸化合物の合成法
    【出願番号】特願2003-198932
    【公開番号】特開2005-35912
    【公開日】平成17年2月10日(公開日:2005-2.10)
    【出願日】2003.7.18
    【出願人】科学技術振興事業団
    【発明者】関根光雄,清尾康志,大窪章寛
  • 11.【発明の名称】オリゴヌクレオチド合成法
    【出願番号】特願2002−264099
    【公開番号】特開2004−99532
    【公開日】平成16年4月2日
    【出願日】平成14年9月10日
    【出願人】シグマジェノシスジャパン(株)
    【発明者】関根 光雄, 清尾 康志, 大窪 章寛
  • 12.【発明の名称】核酸の合成法
    【出願番号】特願2001−162896
    【公開番号】特開2003−2895(P2003−2895A)
    【公開日】平成15年1月8日(2003.1.8)
    【出願日】平成13年5月30日
    【住所又は居所】東京都目黒区大岡山2丁目12番1号
    【発明者】関根 光雄, 清尾 康志, 大窪 章寛
  • 13.【発明の名称】新規ベンジルアジド化合物
    【出願番号】特願平11−51048
    【公開番号】特開2000−247940
    【公開日】平成12年9月12日
    【出願日】平成11年2月26日
    【出願人】三共株式会社
    【発明者】関根 光雄,和田 猛,大窪 章寛

ページ上部へ