Professor, Department of Biological Sciences
PhD 1992, Kyushu University
E-mail: takobaya(at)nig.ac.jp
Office: Institute of Molecular and Cellular Bioscience, The University of Tokyo,

Areas of Research: Molecular Biology

Keywords: Genome stability, Cellular senescence, Cell division

Research interest:

Organisms are alternating generations and maintaining their species. During the period, most cells age to die. But some of them, such as germ line and stem cells, survive and maintain their species and individuals. We study how those cells "rejuvenate" and keep the integrity of genome.

Selected publications

  1. Kobayashi
, T. (2014). Ribosomal RNA gene repeats, their stability and cellular senescence. Proc. Japan Acad., Ser. B, 90: 119-129.
  2. Saka, K., Ide, S., Ganley, A.R., and Kobayashi, T.
 (2013). Cellular senescence in yeast is regulated by rDNA noncoding transcription.
 Curr. Biol. 23: 1794-1798. 
  3. Ide, S., Saka, K., and Kobayashi, T.
 (2013). Rtt109 prevents hyper-amplification of ribosomal RNA genes through histone modification in budding yeast. 
PLoS Genet. 9: e1003410.
  4. Ide, S., Miyazaki, T., Maki, H., and Kobayashi, T. (2010). Abundance of ribosomal RNA gene copies maintains genome integrity. Science 327: 693–696.
  5. Ganley, A.R.D., Ide, S., Saka, K., and Kobayashi, T. (2009). The effect of replication initiation on gene amplification in the rDNA and its relationship to aging. Mol. Cell 35: 683-693.
  6. Kobayashi, T. and Ganley, A.R.D. (2005). Recombination regulation by transcription-induced cohesin dissociation in rDNA repeats. Science 309: 1581-1584.
  7. Ganley, A.R.D., Hayashi, K., Horiuchi, T. and Kobayashi, T. (2005). Identifying gene-independent noncoding functional elements in the yeast ribosomal DNA by phylogenetic footprinting. Proc. Natl. Acad. Sci. USA 102, 11787-11792.
  8. Kobayashi, T., Horiuchi, T., Tongaonkar, P., Vu, L., and Nomura, M. (2004). SIR2 regulates recombination between different rDNA repeats, but not recombination within individual rRNA genes in yeast. Cell 117, 441-453.
  9. Kobayashi, T. (2003). The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Mol. Cell. Biol. 23, 9178-9188.
  10. Takeuchi, Y., Horiuch, T. and Kobayashi, T. (2003). Transcription-dependent recombination and the role of fork collision in yeast rDNA. Genes Dev. 17, 1497-1506.
  11. Kobayashi, T., Heck, D.J., Nomura, M., and Horiuch, T. (1998). Expansion and contraction of ribosomal DNA repeats in Saccharomyces cerevisiae: requirement of replication fork blocking (Fob1) protein and the role of RNA polymerase I. Genes Dev. 12, 3821-3830.

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